Biotechnology And Its Applications MCQs For NEET

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NEET Biology Biotechnology And Its Applications Multiple Choice Questions

Question 1. How many recombinant therapeutics have been approved for human use the world over?

  1. 12
  2. 30
  3. 20
  4. 18

Answer: 2. 30

Question 2. Which of the following techniques serve the purpose of early diagnosis?

  1. Recombinant DNA technology
  2. PCR
  3. ELISA
  1. 1 only
  2. 1 and 3 only
  3. 1 and 2 only
  4. 2 and 3.

Answer: 4. 2 and 3

Question 3. Which of the following techniques is based upon the principle of antigen-antibody interaction?

  1. PCR
  2. ELISA
  3. Recombinant DNA technology
  4. RNA interference.

Answer: 2. ELISA

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Question 4. Which of the following transgenic protein products has been used to treat emphysema?

  1. α-1-antitrypsin
  2. a-Lactalbuniin
  3. Cry protein
  4. C-peptide.

Answer: 1. α-1-antitrypsin

Biotechnology And Its Applications MCQs For NEET

Biotechnology and its Applications Zoology NEET Question 5. The Bt toxin gene has been cloned from the bacteria and expressed in plants to provide resistance to insects without the need for insecticides. Select these resistant plants from the given list.

  1. Cotton
  2. Corn
  3. Rice
  4. Tomato
  5. Potato
  6. Soyabean
  1. 1, 2, 3 and 4
  2. 2, 3, 4 and 5
  3. 1, 2, 5 and 6
  4. All of these

Answer: 4. All of these

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Question 6. Which of the following is/are correct about the process of RNA interference?

  1. This is used to prevent the infestation of protozoans.
  2. It takes place in some eukaryotic and all prokaryotic organisms as a method of cellular defense.
  3. The method involves silencing a specific mRNA due to a complementary dsRNA molecule.
  4. It is a novel strategy to produce pest-resistant plants
  1. 3 and 4
  2. 2, 3 and 4
  3. 1 and 3
  4. 3 only

Answer: 1. 3 and 4

Question 7. Which ingredient was present in high concentrations in genetically modified (GM) rice as compared to the usual rice?

  1. Protein
  2. Carbohydrates
  3. Na+ ions
  4. Vitamin A.

Answer: 4. Vitamin A.

Question 8. Animals that have had their DNA manipulated to possess and express an extra gene are known as:

  1. Foreign animals
  2. Superior animals
  3. Transgenic animals
  4. Intergenic animals.

Answer: 3. Transgenic animals

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Biotechnology and its Applications Zoology NEET Question 9. About 95 percent of all existing transgenic animals are

  1. Rabbits
  2. Pigs
  3. Crovs
  4. Mice

Answer: 4. Mice

Question 10. Today, transgenic models exist for many human diseases which include:

  1. Cancer
  2. Cystic fibrosis
  3. Rheumatoid arthritis
  4. Alzheimer’s disease.
  1. 1 and 3 only
  2. 2 and 3 only
  3. 1, 2 and 3 only
  4. 1, 2, 3 and 4.

Answer: 4. 1, 2, 3 and 4.

Question 11. Which of the following is not a true statement with respect to Bt cotton?

  1. Bt toxin is produced by a bacterium Hticillus tliuringH’nsix
  2. It is an example of bio-pesticide
  3. Bt toxin gene has been cloned in plants to provide resistance to insects
  4. Bt cotton could decrease the amount of pesticide used.

Answer: 3. Bt toxin gene has been cloned in plants to provide resistance to insects

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Question 12. Match the following genes in Column-A with the insect that can be protected from with their coded protein in Column-B.

Biotechnology And Its Applications Match The Genes With Insect That Can Be Protected From With Their Coded Protein

  1. 1(A), 2(C), 3(B)
  2. 1(B), 2(A), 3(C)
  3. 1(A), 2(B), 3(C)
  4. 1(B), 2(C), 3(A).

Answer: 1. 1(A), 2(C), 3(B)

Question 13. Which protein would you like to be produced by genetic engineering as a cure for diseases like emphysema?

  1. α-1-antitrypsin
  2. Trypsin
  3. Chymotrypsin
  4. All of the above are required.

Answer: 1. α-1-antitrypsin

Question 14. ‘Rosie’ a transgenic cow is known to produce a type of milk that has all the following characteristics except:

  1. Proteimeontent of 2.4 gm/litre
  2. Has human α-lactalbumin
  3. More balanced diet than normal cow milk for babies
  4. Was produced for the first time in the year 2001.

Answer: 4. Was produced for the first time in the year 2001.

Question 15. According to the latest estimates, how many documented varieties of Basmati rice are grown in India?

  1. 30
  2. 27
  3. 118
  4. 125.

Answer: 2. 27

Question 16. An anti-bacterial compound that prevents mastitis in cows is:

  1. α-1-antitrypsin
  2. Lysostaphin
  3. Lysozyme
  4. Alginate lyase.

Answer: 2. Lysostaphin

 Question 17. Choose the incorrect statement with respect to Bioweapons

  1. They are low-cost weapons
  2. They cause more casualties than conventional weapons
  3. They are extremely difficult to detect
  4. Bacterium E. coli created a later scare in 2001.

Answer: 4. Bacterium E. coli was created later in 2001

Question 18. A set of standards by which a community regulates its behavior and activities in relation to the biological world is termed as:

  1. Biopatent
  2. Biopiracy
  3. Patent
  4. Biocthic.

Answer: 4. Biocthic.

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Question 19. Nexia biotechnologies spliced spider genes into the cells of lactating:

  1. Cow
  2. Sheep
  3. Goat
  4. None of these.

Answer: 3. Goat

Question 20. How many varieties of rice have been estimated to be present in India’?

  1. 2.000
  2. 20,000
  3. 200,000
  4. 2,000,000.

Answer: 3. 200,000

Question 21. The use of bioresources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory’ payment is called:

  1. Bioethics
  2. Biopiracy
  3. Bioterror
  4. Bioweapon.

Answer: 2. Biopiracy

Question 22. Amongst the following which characteristics is not applicable to Bt cotton?

  1. Bt is the abbreviated term for botulinum toxin
  2. Such cotton is resistant to armyworms and beetles
  3. The toxin is activated in the body of the insect
  4. The toxin is coded by a gene called ‘cry’.

Answer: 1. Bt is the abbreviated term for botulinum toxin

Question 23. Which biotechnology company is credited with the synthesis of genetically engineered human insulin for the first time?

  1. Celera genomics
  2. Cipla
  3. Eli Lily
  4. Ranbaxy.

Answer: 3. Eli Lily

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Question 24. Pick the odd one out

  1. DNA micro-injection
  2. RNA interference
  3. Retrovirus-mediated gene transfer
  4. Embryonic stem cell-mediated gene transfer.

Answer: 2. RNA interference

Question 25. In Xenotransplantation, a protein that causes graft rejection comes usually from transgenic

  1. Cow
  2. Mice
  3. Pig
  4. Sheep.

Answer: 3. Pig

Question 26. Transgenics have provided many pharmaceuticals in their milk, for the treatment of diseases. Which of the following has not been a successful story?

  1. Phenylketonuria
  2. SCID
  3. Emphysema (hereditary)
  4. CFTR.

Answer: 2. SCID

Question 27. Which is not true with respect to transgenic animals and their contribution to human welfare?

  1. Transgenic mice are being tested to ensure the safety of the polio vaccine
  2. Rosie’s milk contained the human gene insulin
  3. Transgenic cows produce milk with less lactose
  4. Transgenic sheep grow more wool.

Answer: 2. Rosie’s milk contained the human gene insulin

Question 28. The functional ADA cDNA can be introduced into the cells of the patient receiving gene therapy by using a vector constituted by

  1. E. coli
  2. Reovirus
  3. Retrovirus
  4. Agrobacterium.

Answer: 3. Retrovirus

Question 29. Which variety office was patented by a U.S. company even though the highest number of varieties of this rice is found in India?

  1. Sharbati Sonora
  2. Co-667
  3. Basmati
  4. Lerma Roja.

Answer: 3. Basmati

Question 30. Which step of Government of India has taken to cater to the requirement of patent terms and other emergency provisions in this regard?

  1. Biopiracy act
  2. Indian patents bill
  3. RTI act
  4. Negotiable Instruments Act.

Answer: 2. Indian patents bill

Biotechnology And Its Applications Mcq For Neet

Question 31. What is another term used for GMO (Genetically Modified Organisms)?

  1. Cybrid organisms
  2. Geromorphic organisms
  3. Transgenic organisms
  4. Conjoint twins.

Answer: 3. Transgenic organisms

Question 32. Which one of the following can be used as a permanent cure for ADA deficiency?

  1. Bone marrow transplantation upon detection of disorder in adult
  2. Enzyme replacement therapy at any point in life
  3. Both 1 and 2
  4. Gene therapy at early embryonic stages.

Answer: 4. Gene therapy at early embryonic stages.

Question 33. Which of the following is a transgenic product useful for the treatment of Haemophilia?

  1. Factor 8
  2. Antithrombin 2
  3. α-1 -antitrypsin
  4. Lysostaphin.

Answer: 1. Factor 8

Question 34. Who is responsible for obtaining interferons through recombinant DNA technique?

  1. A.R. Bunting
  2. Eli Lily
  3. A. Tiselius.
  4. Charles Weissmann

Answer: 3. A. Tiselius

Question 35. Select the incorrect statement

  1. RNAi silencing takes place in all eukaryotic organisms as a method of cellular defense
  2. RNAi requires silencing of mRNA by binding of complementary ssDNA molecule
  3. Complementary nucleic acid could be from mobile genetic elements (transposons)
  4. Ti plasmid with nematode-specific genes have been used in RNAi.

Answer: 2. RNAi requires silencing of mRNA by binding of complementary ssDNA molecule

Biotechnology and It’s Applications Biology NEET Question 36. Identify the correct match for the given columns.

Biotechnology And Its Applications Identify And Match The Columns

  1. 1(D),2(C), 3(B), 4(A)
  2. 1(A),2(C), 3(D), 4(B)
  3. 1(D),2(B), 3(A), 4(C)
  4. 1(B),2(B), 3(D), 4(A)

Answer: 1. 1(D),2(C), 3(B), 4(A)

Question 37. Rules of conduct that may be used to regulate our activities in relation to the biological world is called

  1. Bioethics
  2. Biowar
  3. Biopatent
  4. Biopiracy.

Answer: 1. Bioethics

Question 38. Transgenic mice are being used to test the safety of:

  1. Polio vaccine
  2. BCG vaccine
  3. Antitrypsin
  4. Hepatitis B vaccine.

Answer: 1. Polio vaccine

Question 39. Which genes encode the protein to control bollworm infection in cotton plants?

  1. Cry 2 Ab
  2. Cry 1 Ac
  3. Both (1) and (3)
  4. Ampr.

Answer: 3. Both (1) and (3)

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Question 40. Which is Incorrect with respect to GM foods?

  1. It contains the protein produced by the transgene in question
  2. GM food contains antibiotic resistance gene itself
  3. The enzyme produced by antibiotic resistance gene will not cause allergies
  4. The bacteria is the gut of humans could be taken by antibiotic resistance genes.

Answer: 3. The enzyme produced by antibiotic resistance gene will not cause allergies

Question 41. Golden rice – a transgenic variety of rice is principally richer than normal rice in:

  1. Cry 1 Ab
  2. Hirudin
  3. TPA
  4. β-carotene.

Answer: 4. β-carotene.

Question 42. Southern blotting can’t be performed without

  1. Restriction endonucleases
  2. Agarose
  3. Monoclonal antibodies
  4. Both (1) and (2).

Answer: 4. Both (1) and (2).

Question 43. Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because:

  1. Bacteria are resistant to the toxin
  2. Toxin exists as a toxoid
  3. Toxins exist as protoxins
  4. Bacteria enclose toxins in a special sac.

Answer: 3. Toxins exist as protoxins

Question 44. What is the permanent curve of adenosine deaminase (ADA) deficiency in children?

  1. Bone marrow transplantation.
  2. Enzyme replacement therapy in which functional ADA is given to the patient by injection.
  3. Infusion of genetically engineered lymphocytes (in which functional ADA-cDNA is introduced) into the patient’s body.
  4. Introduction of gene isolated from bone marrow cells producing ADA, into cells at early embryonic stages.

Answer: 4. Introduction of gene isolated from bone marrow cells producing ADA, into cells at early embryonic stages.

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Question 45. Which gene controls the transcription of chain A and chain B required for humulin synthesis in E. coli?

  1. β-lactamase
  2. β-galactosidase
  3. Poly galacturonic
  4. Chitinase.

Answer: 3. Poly galacturonic

Question 46. Transgenic Brassica napus has been used for the synthesis of

  1. Hirudin
  2. Heparin
  3. Polygalacturonase
  4. Cry protein.

Answer: 1. Hirudin

Question 47. Which of these participates in the manufacturing of dextrans:

  1. Mucor
  2. Lactobacillus
  3. Leuconostoc
  4. Pseudomonas.

Answer: 3. Leuconostoc

Question 48. Which of the following is used for the manufacture of cosmetics?

  1. Serotonin
  2. Cholesterol
  3. Shikonin
  4. Keratin.

Answer: 3. Shikonin

Question 49. The technique by which virus detection can be made both in plants and animals is known as:

  1. Enzyme-linked immunoabsorbent assay (ELISA)
  2. Electrophoresis
  3. Electron microscopy
  4. Immunofluorescence

Answer: 1. Enzyme-linked immunoabsorbent assay (ELISA)

Question 50. Streptokinase (TPA) helps in:

  1. Clearing blood clots
  2. Increasing plasma
  3. Dissolving tissues
  4. Increasing O.P. of the cell.

Answer: 1. Clearing blood clots

Question 51. The enzyme diastase was identified by:

  1. Payen and Persoz
  2. Waksman
  3. Christian Hansen
  4. Alexander Fleming.

Answer: 1. Payen and Persoz

Question 52. The immobilization of the enzyme is achieved by:

  1. Killing the cells without affecting enzymes
  2. Attaching the enzymes covalently to a solid support
  3. Entrapping the living cells in a gel
  4. All of these.

Answer: 4. All of these.

Question 53. Interferons are:

  1. Proteins released in minute quantities by animals or plant cells, on being infected with a virus
  2. Proteins synthesized in large quantities by cells actively engaged in synthesis
  3. Hormones released by cells in small quantities which influence some of the various physiological activities
  4. Defensive mechanism.

Answer: 1. Proteins released in minute quantities by animals or plant cells, on being infected with the virus

Question 54. Magic bullets are:

  1. Interferons
  2. Vaccines
  3. Monoclonal
  4. Antigen-antibody cells, antibodies

Answer: 3. Monoclonal

Question 55. Cheese contains:

  1. Proteins 20-35%, fats 20-3%
  2. Fats 20-35%, protein 20-30%
  3. Proteins double than fats
  4. Fats double proteins.

Answer: 1. Proteins 20-35%, fats 20-3%

Question 56. The hybridoma technique was developed by:

  1. Louis Pasteur
  2. Edward Jenner
  3. Georges Kohler
  4. Burnet. and Caser Milstein

Answer: 3. Georges Kohler

Question 57. Some of the steps involved in the production of humulin are given below. Choose the correct sequence.

  1. Synthesis of the gene (DNA) for human insulin artificially.
  2. Culturing recombinant E. coli in bioreactors.
  3. Purification of humulin.
  4. Insertion of human insulin gene into a plasmid.
  5. Introduction of recombinant plasmid into E. coli.
  6. Extraction of recombinant gene product from E. coli.
  1. 2, 1, 4, 3, 5, 6
  2. 1, 3, 5, 6, 2, 4
  3. 1, 4, 5, 2, 6, 3
  4. 3, 5, 2, 1, 6, 4

Answer: 3. 1, 4, 5, 2, 6, 3

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